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. 2001 Sep 17;194(6):733–746. doi: 10.1084/jem.194.6.733

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© 2001 The Rockefeller University Press

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Surface staining of thymocytes from a RAG-2^−/− mouse that was lethally irradiated and reconstituted with E47^−/− RAG-1^−/− bone marrow 6 wk before analysis. A RAG-deficient host was used because the donor bone marrow was from a mixed genetic background. (A) Surface CD8 and CD4 expression. (B) Surface CD44 and CD25 expression on DN thymocytes. The DN3 cells were found to be ∼3% positive when stained with anti-E47, indicating that the vast majority of the DN thymocytes were donor derived (data not shown). 4.2 × 10⁶ cells were recovered from this thymus, which is within the range obtained from irradiated hosts reconstituted with E2A-deficient bone marrow (data not shown).

Surface staining of thymocytes from a RAG-2^−/− mouse that was lethally irradiated and reconstituted with E47^−/− RAG-1^−/− bone marrow 6 wk before analysis. A RAG-deficient host was used because the donor bone marrow was from a mixed genetic background. (A) Surface CD8 and CD4 expression. (B) Surface CD44 and CD25 expression on DN thymocytes. The DN3 cells were found to be ∼3% positive when stained with anti-E47, indicating that the vast majority of the DN thymocytes were donor derived (data not shown). 4.2 × 10⁶ cells were recovered from this thymus, which is within the range obtained from irradiated hosts reconstituted with E2A-deficient bone marrow (data not shown).