Figure 3.

TNF-α, IL-6, IFN-α, and IL-12 production by monocytes (Mono), CD11c⁺ imDCs, and plasmacytoid pre-DCs (pDC) stimulated with microbial molecules that trigger different TLR signaling. The three cell populations were cultured with 10 μg/ml PGN, 10 μg/ml LTA, 10 μg/ml LPS, 50 μg/ml poly I:C, or with 5 μM CpG-ODN AAC-30 for 24 h, and the concentrations of cytokines in the supernatants were measured by ELISA. The data shown are representative of four or five experiments.