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Expression of CD4U but not CD4U2/6 causes spontaneous apoptosis in HeLa cells. Inducible HeLa-CD4U and CD4U2/6 cell lines were cultured after the removal of Dox for the times indicated. The cells were then evaluated for induction of apoptosis by staining with PE-conjugated annexin V, followed by flow cytometry. Error bars reflect SDs from three independent experiments.
