FIG. 3.

Western blot analysis for expression of IL-1α protein in ileal tissues of cattle (n = 3) infected with M. avium subsp. paratuberculosis (infected tissues; I1 to I3) and uninfected control cattle (control tissues; C1 to C3). Proteins were separated by SDS-PAGE and Western blot analysis was performed as described in Materials and Methods. Final autoradiograms were analyzed by densitometry and background-subtracted intensities were obtained. Relative intensities were combined within a group and used to calculate mean relative intensities for each infection group as well as standard error of the means.