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. 2001 Nov 5;194(9):1339–1348. doi: 10.1084/jem.194.9.1339

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Copyright © 2001, The Rockefeller University Press

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Figure 1. — Generation of tumor cell lines expressing B7H-GFP fusion protein. (A) Intensity of green fluorescence in J558 cells transfected with vector alone (J558-Neo) or B7H-GFP. Profiles of three independent clones were presented. These clones were cultured separately, mixed at an 1:1:1 ratio, and used in all subsequent experiments. (B) ICOSIg binds to J558-B7H, but not to J558-Neo. The J558-transfectants were incubated with either human IgG1Fc or ICOSIg (50 μg/ml), and the binding was measured by flow cytometry. (C) Susceptibility of J558-B7H, J558-Neo, and J558-B7 to transgenic P1CTL, which had been stimulated in vitro for 4 d with P1A peptide (0.1 μg/ml). Two independent experiments were presented. Unpulsed or P1A-pulsed P388D1(H-2^d) target cells were used as negative and positive controls, respectively.

Figure 1. — Generation of tumor cell lines expressing B7H-GFP fusion protein. (A) Intensity of green fluorescence in J558 cells transfected with vector alone (J558-Neo) or B7H-GFP. Profiles of three independent clones were presented. These clones were cultured separately, mixed at an 1:1:1 ratio, and used in all subsequent experiments. (B) ICOSIg binds to J558-B7H, but not to J558-Neo. The J558-transfectants were incubated with either human IgG1Fc or ICOSIg (50 μg/ml), and the binding was measured by flow cytometry. (C) Susceptibility of J558-B7H, J558-Neo, and J558-B7 to transgenic P1CTL, which had been stimulated in vitro for 4 d with P1A peptide (0.1 μg/ml). Two independent experiments were presented. Unpulsed or P1A-pulsed P388D1(H-2^d) target cells were used as negative and positive controls, respectively.