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. 2001 Nov 5;194(9):1339–1348. doi: 10.1084/jem.194.9.1339

Figure 2.

Figure 2.

B7H expression reduced tumorigenicity of the J558 cells in immune competent mice and induced protection to subsequent challenge with the parental tumors. (A) Tumor incidence of J558-Neo (n = 6) and J558-B7H (n = 10) in immune competent mice. Data are representative of four independent experiments. In total, 24/26 mice receiving J558-Neo cells developed tumors, while 23/39 mice receiving the J558-B7H cells developed tumors. (B) J558-B7H and J558-Neo tumors grew at a similar rate in syngeneic RAG-2−/− mice (n = 7). Data shown are representative of four independent experiments, which in total involved 23 mice per group. All mice developed tumors within 2 wk. Syngeneic BALB/c or BALB/crag2−/− mice received 5 × 106 tumor cells in the flank, and the tumor incidences were determined by physical examination. (C and D) Mice that rejected J558-B7H tumors were immune to subsequent challenge with parental J558 cells. Syngeneic BALB/c mice that had rejected J558-B7H tumors and naive control mice were challenged with 5 × 106 J558 cells at the opposite flank. The tumor incidence (C) and growth kinetics (D) were measured by physical examination. This experiment was repeated twice with similar results.