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. 2001 Nov 5;194(9):1339–1348. doi: 10.1084/jem.194.9.1339

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Copyright © 2001, The Rockefeller University Press

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Figure 5. — Expression of B7H on the tumor cells promotes T cell clonal expansion. Purified CD8 T cells from P1CTL transgenic mice were labeled with CFSE and adoptively transferred into RAG-2^−/− BALB/c mice bearing either J558-Neo or J558-B7H tumors, or mice that received no tumor cells as control. At 36, 60, and 84 h after the adoptive transfer, T cells were isolated from either spleen or tumors and were analyzed for the number of divisions that they had undergone. FACS^® profiles of CFSE intensity of gated CD8⁺Vα8⁺ T cells isolated from spleen were presented. The percent of the CD8⁺Va8⁺ cells were shown in the panels.