Table I.
Reactive Cell Content, Expression of Endothelial Adhesion Molecules, and Production of Cytokines, Angiogenic Factors, and Mediators in Tumors Growing in Balb-neuT Mice
| MSA | IL-12 | Neu/H-2q cellsplus IL-12 | |
|---|---|---|---|
| Reactive cells a | |||
| Dendritic cells | 1.4 ± 0.7 | 11.0 ± 3.2b | 9.3 ± 2.8b |
| Macrophages | 3.1 ± 1.3 | 4.2 ± 1.8 | 22.1 ± 4.2c |
| PMN | 4.5 ± 2.0 | 4.6 ± 2.3 | 23.3 ± 5.2c |
| CD8+ lymphocytes | 1.3 ± 0.9 | 6.7 ± 2.4b | 12.7 ± 3.7c |
| CD4+ lymphocytes | 0.0 | 1.3 ± 0.4b | 2.0 ± 0.6 |
| NK cells | 5.1 ± 1.9 | 14.6 ± 3.3b | 11.2 ± 3.5b |
| Microvessel count | 14.4 ± 2.2 | 9.0 ± 1.8b | 6.1 ± 1.7b |
| Endothelial adhesion molecules d | |||
| ELAM-1 | – | – | + |
| ICAM-1 | + | + | ++ |
| VCAM-1 | – | – | ± |
| Cytokines andmediators d | |||
| IL-1β | – | + | ++ |
| TNF-α | ± | ± | + |
| IFN-γ | – | ± | + |
| MCP-1 | – | + | ++ |
| MIP-2 | ± | ± | + |
| RANTES | – | – | ± |
| IP-10 | + | ++ | ++ |
| MIG | + | ++ | ++ |
| iNOS | – | + | + |
| Angiogenic factors d | |||
| VEGF | ± | ± | ± |
| bFGF | + | + | + |
Cell counts performed in ten randomly chosen high-power fields per sample. Results are mean ± SD of positive cell per field.
Significantly different (P < 0.001) from corresponding values in MSA control mice.
Significantly different (P < 0.001) from corresponding values in MSA control and IL-12–treated mice.
The expression of adhesion molecules, cytokines, mediators, and angiogenic factors was defined as absent (-), scarcely (±), moderately (+), and strongly (++) present on cryostat sections decorated with the antibody.
bFGF, basic fibroblast growth factor; ELAM, endothelial leukocyte adhesion molecule E-selectin; ICAM, intercellular adhesion molecule; iNOS, inducible nitric oxide synthase; MCP, monocyte chemotactic protein; VCAM, vascular cell adhesion molecule; VEGF, vascular endothelial cell growth factor.