Table I.

Comparison of Monocyte and Naive T Cell Migration to Resting and Inflamed PLNs

Cell type	Monocytes		Naive T cells
PLNs	Contralateral	Inflamed	Contralateral	Inflamed
Total no. of cells/PLN (× 106)	3.43 ± 1.21	10.58 ± 2.10	0.89 ± 0.17	10.31 ± 2.27
Total no. of homed GFPMED cells/PLN	37 ± 18	173 ± 48	1,712 ± 229	19,228 ± 3,837
Recruitment rate (× h−1 × 10−6 cells injected)	28.9 ± 12.9	164 ± 53	107 ± 14	1,202 ± 240
GFPMED cells/μl blood	4.4 ± 0.7		9.7 ± 2.1

Comparison of T cell and monocyte recruitment efficiency to resting and inflamed PLNs. Recipient mice with unilateral flank skin inflammation (5–7 d) were injected with 5 × 10⁶ donor cells from either CX3CR^+/GFP mice (PBMCs containing ∼10% GFP⁺ cells, half of which were GFPMED monocytes) or T-GFP mice (LN cells containing ∼80% GFP⁺ naive T cells). Peripheral blood and the subiliac LNs contralateral and ipsilateral to the inflamed tissue were harvested 4 h later and analyzed for the presence of GFP⁺ cells and the rate of cell recruitment was calculated. In animals that received CX3CR^+/GFP cells the frequency and rate of recruitment were also determined for the GFP^MED subset (shown in parenthesis). Data are shown as mean ± SEM of five experiments in each group.