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. 2001 Feb 5;152(3):645–650. doi: 10.1083/jcb.152.3.645

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Targeting of the mouse keratin 10 gene. (A) Genomic organization of the mouse keratin 10 locus. Exon 1 contains codon 154 encoding arginine (R154). (B) The targeting vector contains a C to T mutation encoding cysteine (C154) which also destroys an AciI restriction site. (C) Recombinant mut^neo allele after homologous recombination in ES cells. (D) Recombinant mut^loxP allele after Cre-mediated excision of PGKneo. 5′ and 3′: probes used for Southern blot analysis. R, EcoRV; B, BglII; X, XhoI; S, ScaI; A, ApaI; N, NotI; K, KpnI.