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. 2001 Feb 5;152(3):503–518. doi: 10.1083/jcb.152.3.503

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Erv41p is coimmunoprecipitated with HA-tagged Erv46p. Microsomes were prepared from FY833 (− HA-Erv46p) and CBY767 (+ HA-Erv46p) cells grown in YP with 2% galactose and 0.2% glucose and solubilized with buffer containing 2% Triton X-100 (T). The soluble extracts (S) were incubated in absence (− anti-HA ab) or presence (+ anti-HA ab) of the monoclonal anti-HA antibody. Precipitates were resolved on a 12.5% polyacrylamide gel and immunoblotted for Sec23p, Erv46p, Erv41p, and Erv25p.