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. 2001 Feb 5;152(3):503–518. doi: 10.1083/jcb.152.3.503

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Immunoblot analysis of wild-type, erv41Δ, and erv46Δ deletion and overproducing strains. FY834 wild-type (wt), erv46Δ (46Δ, CBY799), and erv41Δ (41Δ, CBY797) strains were grown in YPD medium. Heterozygous diploid strains expressing NH₂ terminally 3HA-tagged Erv46p (46/HA46, CBY767) or Erv41p (41/HA41, CBY782) were grown in YP with 1.5% galactose and 0.5% glucose. erv14Δ erv46Δ deletion strains carrying pRS426-ERV46 plasmid (2μ 46, CBY836) or pRS424-ERV41 plasmid (2μ 41, CBY847) were grown in minimal media lacking uracil or tryptophan, respectively. Membrane fractions were resolved on a 12.5% polyacrylamide gel and immunoblotted with an anti-Kar2p antiserum as loading control and with polyclonal antisera raised against Erv46p or Erv41p.