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. 2001 Feb 5;152(3):503–518. doi: 10.1083/jcb.152.3.503

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Erv46p and Erv41p are integral membrane proteins. (A) Hydrophilicity plots according to Kyte and Doolittle 1982 with a window size of 7. The schematic representations of the polypeptide chains indicate the positions of the putative transmembrane domains (TM1 and TM2) predicted by the HMMTOP program (Tusnady and Simon 1998), the region containing conserved cysteine residues (Cys) and the KKXX motif (black dot). (B) Semiintact FY834 cells were treated with buffer (Materials and Methods), buffer containing 1% Triton X-100 (TX100), or 0.1 M Na₂CO₃ (pH 11) and centrifuged at 100,000 g. Totals before centrifugation (T), supernatant (S), and pellet (P) fractions were analyzed on a 12.5% polyacrylamide gel and immunoblotted for Sec23p (peripheral membrane protein), Erv46p, Erv41p, and Bos1p (integral membrane protein).