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. 2001 Feb 5;152(3):503–518. doi: 10.1083/jcb.152.3.503

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Genetic experiments with erv41Δ and erv46Δ strains. (A) An erv14Δ strain (CBY358) was mated with an erv46Δ strain (CBY799), and spores were dissected on a YPD plate. Spores that germinated and grew slower were shown to carry both deletions. (B) Cold sensitivity of erv14Δ, erv41Δ, and erv46Δ strains. Wild-type (FY834), erv46Δ (CBY799), erv41Δ (CBY797), erv14Δ (CBY356), erv14Δ erv46Δ (CBY823), erv14Δ erv41Δ (CBY825), erv41Δ erv46Δ (CBY795), and erv14Δ erv41Δ erv46Δ (CBY894) strains were grown to saturation in YPD, adjusted to an OD₆₀₀ of 3.0, and 5 μl of a 10-fold dilution series were spotted onto YPD plates. (C) Effects of erv41Δ and erv46Δ mutations on the ypt1-3 mutation. Wild-type (FY834), ypt1-3 (CBY829), erv46Δ ypt1-3 (CBY843), erv41Δ ypt1-3 (CBY841), erv41Δ erv46Δ ypt1-3 (CBY845), and erv41Δ erv46Δ (CBY795) cells were spotted on YPD plates as in B.