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. 2001 Feb 5;152(3):503–518. doi: 10.1083/jcb.152.3.503

Table 2.

Primer Sequences

Primer Sequence
YML067c-NotI 5′-ATAAGAATGCGGCCGCAACTCCCCTTCTCCAGACTTG-3′
YML067c-BamHI 5′-CGCGGATCCGCGAAGTATGCGGGACGCTG-3′
YAL042w-NotI 5′-ATAAGAATGCGGCCGCCTGCAGATGACATTGCGCTGC-3′
YAL042w-BamHI 5′-CGCGGATCCGCCATGATCTCTCGGGTTGG-3′
YML067c-pQE-F 5′-CGCGGATCCACGAAATGTGATTGGTTGC-3′
YML067c-pQE-R 5′-CCCAAGCTTCACCTTTAGCAGCGACATC-3′
YAL042w-pQE-F 5′-CGCGGATCCTGTGACCTGGTGAATCTCG-3′
YAL042w-pQE-R 5′-CCCAAGCTTCCTGTCCGGGAACACC-3′
OCH1-pQE-F 5′-CGCGGATCCGATCCAAGATTTGAAGAAG-3′
OCH1-pQE-R 5′-CCCAAGCTTGGGTATGATGAAAGGAGAG-3′
YML067c-KO-F 5′-TGCTTTTGCTGTAACGTACAGGCAGAGACTTCAGGAGTATATAGGCCTCCTCTAGTACACTC-3′
YML067c-KO-R 5′-GAAACGTTATCTTGGGTATACTGCATTCTCTTTTCTTTTACATATGCGCGCCTCGTTCAGAATG-3′
HIS3 5′-GCCTCATCCAAAGGCGC-3′
YAL042w-F4 5′-CGCCATTATAAAGGAAAGCTAGTTTTATGTCTCGTATACAGAATTCGAGCTCGTTTAAAC-3′
YAL042w-R3 5′-TAGCGAATGCGTCCAGCGACAGCAACGTGGACCTTTTCATGCACTGAGCAGCGTAATCTG-3′
YML067c-F4 5′-GATATTATCAATGTTCTATTCTTACTGAGAAAAGCGTTCCGAATTCGAGCTCGTTTAAAC-3′
YML067c-R3 5′-ATAACATACGAAACGCATCAAATGTCTTCAATCCTGCCATGCACTGAGCAGCGTAATCTG-3′
YGR284c-F4 5′-ACGAAAGATCAAAGGTGTCCTTATTTACTTACAATAGCTGGAATTCGAGCTCGTTTAAAC-3′
YGR284c-R3 5′-GGCATACCGCCAAAATTTCCAATAGGTCCTCTGTAAGACATGCACTGAGCAGCGTAATCTG-3′
MHF2 5′-TGGCTTCATTTGGCAAAATGTTCTAATGTGGTTAATGGGTCGGATCCCCGGGTTAATTAA-3′
MHR1 5′-GCATGAAATTAAATCCTCTCTTTGATCTCTTCAATCAAGAGAATTCGAGCTCGTTTAAAC-3′