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. 2001 Feb 5;152(3):607–620. doi: 10.1083/jcb.152.3.607

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Hsc70 ATPase domain mutants inhibit hsc70-mediated clathrin uncoating. CCVs were incubated in the presence of wild-type and mutant hsc70 proteins. (A) Shown is a Coomassie blue–stained gel of clathrin released into the 100,000-g supernatant. The final μM concentration of recombinant protein used is indicated in parenthesis. (B) The uncoating reaction mediated by hsc70_Native (0.4 μM) was assayed in the presence of increasing hsc70 mutant concentration. The error bars reflect the SD from three independent experiments. WT, wild type.