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. 2001 Feb 5;152(3):607–620. doi: 10.1083/jcb.152.3.607

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Internalized Tfn exhibits prolonged trafficking through punctate structures in hsc70_D199S- and hsc70_T204V-expressing cells as visualized by epifluorescent microscopy. A 5-min pulse of Alexa₄₈₈-Tfn (0 min) was chased with unlabeled Tfn and followed for 5, 10, 20, and 30 min. Cells were fixed and processed for fluorescence microscopy as described in Materials and Methods. All images were collected with 2-s exposures. WT, wild type.