Figure 2.

Cell detachment sensitizes HUVECs to Fas-mediated apoptosis. The ability of agonistic anti-Fas antibody (CH11) to induce apoptosis was analyzed in attached and detached HUVECs. The cells were incubated with or without 1 μg/ml of CH11 for 12 h in the presence or absence of blocking anti–Fas-L antibody (NOK-2), and apoptosis was determined by DNA fragmentation analysis. Bars indicate SD in a representative experiment done in triplicate.