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. 2001 Feb 5;152(3):633–644. doi: 10.1083/jcb.152.3.633

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Cell detachment upregulates Fas expression in HUVECs. (A) Detachment induces cell surface levels of Fas. HUVECs were kept adherent or in suspension for 12 h. FACS^® analysis with anti-Fas antibody (UB2) was carried out as described in Materials and Methods. (B) Cell detachment increases the mRNA levels of Fas. HUVECs were kept adherent or in suspension for the indicated times, and Fas mRNA levels were determined by an RT-PCR analysis as described in Materials and Methods.

Cell detachment upregulates Fas expression in HUVECs. (A) Detachment induces cell surface levels of Fas. HUVECs were kept adherent or in suspension for 12 h. FACS^® analysis with anti-Fas antibody (UB2) was carried out as described in Materials and Methods. (B) Cell detachment increases the mRNA levels of Fas. HUVECs were kept adherent or in suspension for the indicated times, and Fas mRNA levels were determined by an RT-PCR analysis as described in Materials and Methods.