Figure 7.

Ectopic activation of the PI 3′-kinase/Akt and Erk pathways prevents anoikis in HUVECs. HUVECs were cotransfected with an empty vector (control) or with plasmids encoding activated forms of PI 3′-kinase (p110*), Akt (Myr-Akt), and c-Raf-1 (Raf-CAAX), together with a plasmid coding for GFP. After transfection, the cells were kept adherent or in suspension for 24 h. Apoptosis was determined by using FACS^® in the double positive cell population for GFP and propidium iodide as described above. Bars indicate SD in an experiment done in triplicate.