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. 2002 Jan 7;195(1):99–111. doi: 10.1084/jem.20001858

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — sHA induces phenotypic and functional maturation of human and mouse DCs. Human monocyte–derived day 4 DCs (A and B) or murine day 6 bone marrow–derived DCs (C and D) were used after 48-h incubation with 20 μg/ml sHA (bold line), 50 μg/ml HMW-HA (solid line), 100 ng/ml LPS (dotted line), or left untreated (broken line). (A and C) DCs were stained with mAbs directed against MHC class II (top) or B7–2 (CD86; lower) and analyzed by flow cytometry. A representative of five independent experiments is shown. (B and D) The cells were coincubated for 4 d with 10⁵ alloreactive T cells at a DC:TC ratio of 1:20. T cell proliferation was determined on day 5 by addition of 1 μCi of ³[H]thymidine for the final 18 h. Results are shown in counts per minute (CPM) ± SD of triplicate wells. * P > 0.001 compared with untreated DCs (−). A representative of four independent experiments is shown.