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. 2002 Jan 7;195(1):99–111. doi: 10.1084/jem.20001858

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — sHA-mediated DC maturation requires p38-MAPK and P42/44-MAPK phosphorylation. (A and B) Human monocyte–derived day 4 DCs were treated with 50 μg/ml sHA, 100 μg/ml HMW-HA, or 100 ng/ml LPS for the times indicated. Western immunoblotting was performed using mAbs specific for the phosphorylated kinase (top) or total kinase content of the cells (bottom). Shown are blots for (A) p38 MAPK and (B) p42/44 MAPK. (C) Supernatants of DCs stimulated with 30 μg/ml sHA or 1 ng/ml LPS in the presence of the indicated concentrations of the MAPK inhibitors PD98059 (p38), SB 203580 (p42/44), Herbimycin A (src-like tyrosine kinases), or Wortmannin (PI3-kinase) were harvested after 7 h and screened for their TNF-α content by ELISA. Data represent the mean TNF-α release of triplicate wells; pg/mg total protein ± SD.