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. 2002 Jan 7;195(1):99–111. doi: 10.1084/jem.20001858

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Copyright © 2002, The Rockefeller University Press

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Figure 7. — sHA-mediated DC stimulation is not dependent on TLR-2 expression. Day 6 bone marrow–derived DCs from C57BL/6 or C57BL/6TLR-2^−/− were incubated for 48 h with the indicated concentrations of highly purified LPS from Salmonella abortus equi or sHA. (A) The cell free supernatants were screened for their TNF-α content by ELISA. Data represent the mean TNF-α release of triplicate wells; pg/mg total protein ± SD. (B) DCs were treated for 48 h with the same substances described in A, washed, and coincubated for 4 d with 10⁵ alloreactive T cells at a DC/TC ratio of 1:20. T cell proliferation was determined on day 5 by addition of 1 μCi of ³[H]thymidine for the final 18 h. Results are shown in counts per minute (CPM) ± SD of triplicate wells.

Figure 7. — sHA-mediated DC stimulation is not dependent on TLR-2 expression. Day 6 bone marrow–derived DCs from C57BL/6 or C57BL/6TLR-2^−/− were incubated for 48 h with the indicated concentrations of highly purified LPS from Salmonella abortus equi or sHA. (A) The cell free supernatants were screened for their TNF-α content by ELISA. Data represent the mean TNF-α release of triplicate wells; pg/mg total protein ± SD. (B) DCs were treated for 48 h with the same substances described in A, washed, and coincubated for 4 d with 10⁵ alloreactive T cells at a DC/TC ratio of 1:20. T cell proliferation was determined on day 5 by addition of 1 μCi of ³[H]thymidine for the final 18 h. Results are shown in counts per minute (CPM) ± SD of triplicate wells.