Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Jan 7;195(1):113–123. doi: 10.1084/jem.20010956

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — PD5-specific CD8⁺ T cells from in vitro–cultured PBMCs. PBMCs from HLA-A2⁺ donors were cocultured with PD5-loaded APCs (autologous immature DCs) for 12 d, then restimulated with PD5 or a control peptide in the presence of brefeldin A, followed by intracellular staining for IFN-γ. (A) IFN-γ staining of samples from two PBC patients, PBC3 and PBC10, and two control donors, CL1 (alcoholic liver disease) and H1 (a healthy donor). Displayed in the dot plots are cells gated for lymphocyte population by forward-scattering and side-scattering and the CD4⁻ population. The cells within the box are considered IFN-γ⁺. The number next to the box is the percentage of IFN-γ⁺ cells in the CD8⁺ T cell population. (B) Frequency of PD5-specific CD8⁺ T cells in PD5-stimulated PBMC cultures derived from all 20 donors. The cut off value for a positive response was determined as 0.150%, or 3 SD above the mean percentage of IFN-γ producing cells in all 20 samples restimulated with the control peptide. A significant number of IFN-γ–producing cells were detected after restimulation with PD5 in 10/12 (83%) PBC patients but in 0/8 control individuals (P < 0.0007).

Figure 1. — PD5-specific CD8⁺ T cells from in vitro–cultured PBMCs. PBMCs from HLA-A2⁺ donors were cocultured with PD5-loaded APCs (autologous immature DCs) for 12 d, then restimulated with PD5 or a control peptide in the presence of brefeldin A, followed by intracellular staining for IFN-γ. (A) IFN-γ staining of samples from two PBC patients, PBC3 and PBC10, and two control donors, CL1 (alcoholic liver disease) and H1 (a healthy donor). Displayed in the dot plots are cells gated for lymphocyte population by forward-scattering and side-scattering and the CD4⁻ population. The cells within the box are considered IFN-γ⁺. The number next to the box is the percentage of IFN-γ⁺ cells in the CD8⁺ T cell population. (B) Frequency of PD5-specific CD8⁺ T cells in PD5-stimulated PBMC cultures derived from all 20 donors. The cut off value for a positive response was determined as 0.150%, or 3 SD above the mean percentage of IFN-γ producing cells in all 20 samples restimulated with the control peptide. A significant number of IFN-γ–producing cells were detected after restimulation with PD5 in 10/12 (83%) PBC patients but in 0/8 control individuals (P < 0.0007).