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. 2002 Jan 7;195(1):113–123. doi: 10.1084/jem.20010956

Figure 4.

Figure 4.

Figure 4.

Figure 4.

Induction of PD5-specific CTL lines with peptide, exogenous rPDC-E2 protein, or rPDC-E2 complexed with specific Abs. The cytotoxicity of CTL lines generated with the different Ags was tested against autologous BCL targets loaded with PD5 or control peptide at an E/T ratio of 40. (A) PBMCs from patient PBC10 were cocultured for 12 d with APCs loaded with rPDC-E2 protein or PD5 peptide at serial concentrations as indicated. (B) PBMCs from patient PBC9 were cocultured for 12 d with APCs loaded with serial concentrations of rPDC-E2 protein (as indicated) mixed with either human anti–PDC-E2 mAb, a control mAb, control ICs, or F(ab)′2 fragment of the human anti–PDC-E2 mAb. (C) PBMCs from patient PBC11 were cocultured for 12 d with APCs loaded with serial concentrations of rPDC-E2 protein (as indicated) mixed with affinity purified autoAbs from PBC sera or control Abs.