Figure 4.
CD81 inhibits IL2 activation of NK cells and TCR-γδ+ T cells. Purified NK cells or TCR-γδ T cells were cultured in wells (105 cells per well) either untreated or precoated with control IgG1 antibody (5 μg/ml) or anti-CD81 (5 μg/ml). Each culture contained 200 μl of medium with or without supplemented IL-2 (100 U/ml). Supernatants were collected after 18 h and assessed for IFN-γ levels by ELISA. The levels of IFN-γ are presented as mean (pg/ml) ± SD of duplicate samples. Data are representative of three independent experiments using different donors.