Table II.
Histological Characterization of Inflammatory Lesions in DO11.RAG-2−/−/RAG-2−/− Recipients Colonized with E. coli-OVA versus E. coli-TET
| T cells | Intestinal colonization | CD3+/mm2totala | CD3+/mm2IEL | PMN/mm2total | Percentage of eosinophils (PMNs) |
Percentage of BrdU+(CD3) |
|---|---|---|---|---|---|---|
| Naive | pnir15.TET-E. coli | 77.4 ± 44.2b | 5.5 ± 5.0 | 4.0 ± 3.7 | ND | 1.6 ± 1.7 |
| pnir15.OVA-E. coli | 192.3 ± 73.5 | 7.3 ± 3.6 | 8.5 ± 3.8 | 2.4 ± 2.9 | 24.0 ± 5.2 | |
| Th1 | pnir15.TET-E. coli | 203.7 ± 55.8 | 17.9 ± 8.4 | 17.9 ± 8.4 | ND | 2.5 ± 1.8 |
| pnir15.OVA-E. coli | 796.1 ± 190.3 | 66.5 ± 29.3 | 246.0 ± 97.3 | 5.7 ± 4.8 | 34.8 ± 7.6 | |
| Th1 | pnir15.TET-E. coli | 140.1 ± 50.0 | 13.0 ± 8.3 | 0.5 ± 1.0 | ND | 7.5 ± 1.9 |
| pnir15.OVA-E. coli | 385 ± 96.2 | 33.0 ± 5.2 | 496.7 ± 44.7 | 39.6 ± 8.9 | 38.8 ± 9.6 |
RAG-2−/− recipients were reconstituted with 106 CD4+ T cells of the indicated phenotypes that were isolated/generated from DO11.RAG-2−/− donors. 1 d before T cell reconstitution, recipients were colonized with 1010 CFU of the indicated bacteria and maintained with biweekly administration of an additional dose of 1010 CFU bacteria. 8 wk after reconstitution, mice were killed and necropsied.
The total number of CD3+ T cells per mm2 of colonic mucosa, including LP and IEL compartments (see Material and Methods for details).
Data are the means ± SEM from 3–6 animals per group. Values that differed significantly from control (P > 0.05) are indicated in bold font.
ND, not determined.