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. 1997 Feb 3;185(3):439–452. doi: 10.1084/jem.185.3.439

Figure 2.

Figure 2

Cytokines produced by TCL (TCL 3) from B10.A and B10.BR mice are influenced by the source of APC (B10.A, B10.BR, or F1) used for in vitro restimulations. (A–D) After the third, fourth, fifth, ninth, and tenth restimulations of TCL with mMBP Ac1-16 and irradiated splenic APC of the indicated origin, T cells were restimulated for 24 h with peptide and APC of the same origin. Adoptive transfer of EAE was performed after the ninth restimulation (see Table 2). Supernatant fluids were analyzed for cytokines. In Fig. 2 D, the points for the B10.BR TCL-3–B10.A APC are 37 ng/ml of IL-10 after the ninth restimulation and 45 ng/ml of IL-10 after the tenth restimulation. Data presented are average of duplicate wells for each sample. (E) At the ninth restimulation, TCL 3 described for A–D were restimulated for 24 h with peptide and APC of the same (indicated) origin. Supernatant fluids depleted of IL-4 were analyzed for IL-2 levels, using the growth factor dependent cell line HT-2. Data presented are means and standard deviations of four wells for each sample (n = 4).