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. 1997 Feb 3;185(3):471–480. doi: 10.1084/jem.185.3.471

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DAP vSAG7 induces Vβ6-specific expansion in vivo. 3 × 10⁶ DAP vSAG7 cells in 30 μl of PBS were injected in the hind footpad. 5 d later, lymph nodes were taken and CD4⁺ lymphocytes were analyzed by flow cytometry for Vβ3 and Vβ6 expression. Live cells were gated by forward and side scatter. Percentages of Vβ6-expressing cells among CD4+ cells were respectively 11, 25, and 23%, respectively, after DAP DR1, DAP vSAG7, and DAP DR1 vSAG7 injection. This experiment was repeated four times and led to similar results.