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. 1997 Feb 3;185(3):471–480. doi: 10.1084/jem.185.3.471

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DAP vSAG7 can induce a Vβ-specific response of ex vivo derived human PBMCs. 1.5 × 10⁵ mitomycin treated DAP vSAG7 cells were added to 1 × 10⁶ fresh PBMCs in a total volume of 1 ml. After 7 d of culture, cells were harvested and the percentage of CD4⁺ blasts expressing Vβ6.7 and Vβ12 was assayed using flow cytometry. Blasts were gated by forward and side scatter. Results are illustrated as dot plots, with CD4 on the x axis and Vβ on the y axis. This experiment was performed twice and led to similar results.