Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Feb 3;185(3):481–490. doi: 10.1084/jem.185.3.481

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Effects of Bcl-2 overexpression on the TNF-α–activated ceramide pathway. (A) Bcl-2 prevents both TNF-α– and ceramide–induced cell death. TNF-α– and Fas–sensitive MCF-7 cells overexpressing pEBS7 (open bars) or pEBS7/Bcl-2 (filled bars) were treated as in Fig. 3 A and evaluated for cell death by trypan blue at 48 h. (B) Bcl-2 does not prevent the TNF-α–induced elevation in ceramide levels. The Bcl2–overexpressing MCF-7 cells (filled diamonds) and control vector cells (open circles) were treated as in Fig. 3 B, and ceramide levels were measured at the indicated time points.

Effects of Bcl-2 overexpression on the TNF-α–activated ceramide pathway. (A) Bcl-2 prevents both TNF-α– and ceramide–induced cell death. TNF-α– and Fas–sensitive MCF-7 cells overexpressing pEBS7 (open bars) or pEBS7/Bcl-2 (filled bars) were treated as in Fig. 3 A and evaluated for cell death by trypan blue at 48 h. (B) Bcl-2 does not prevent the TNF-α–induced elevation in ceramide levels. The Bcl2–overexpressing MCF-7 cells (filled diamonds) and control vector cells (open circles) were treated as in Fig. 3 B, and ceramide levels were measured at the indicated time points.