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. 1997 Feb 3;185(3):393–404. doi: 10.1084/jem.185.3.393

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Comparison of avidity of CD28 Ig and CTLA-4 Ig binding to immobilized sCD80. CD28 Ig or CTLA-4 Ig were injected at 5 μl/ min (25°C) at the indicated concentration for 6–8 min through FC with either sCD80 (3300 RU) or no protein (Control) immobilized. The responses seen with injection of the three CTLA4 Ig concentrations through the Control FC were indistinguishable when superimposed.