Figure 4.
Egr-1 is required for endogenous NF-κB1 gene expression. (A) Total RNAs were isolated from CEM/CMV control cells or from CEM/AsEgr-1 cells stimulated with either PMA/PHA or TNF-α over a 4 h period. RNAs were detected using a 32P-labeled NF-κB1 specific probe. (B) Total proteins were isolated from CEM/CMV or CEM/AsEgr-1 cells after the addition of either PMA/PHA or TNF-α. Proteins were subjected to PAGE and analyzed using an Egr-1–specific antibody and enhanced chemiluminescent assay. NS, shows the presence of a nonspecific band and demonstrates that equal amounts of protein were loaded in each lane.