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. 1997 Feb 3;185(3):491–498. doi: 10.1084/jem.185.3.491

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Egr-1 is required for endogenous NF-κB1 gene expression. (A) Total RNAs were isolated from CEM/CMV control cells or from CEM/AsEgr-1 cells stimulated with either PMA/PHA or TNF-α over a 4 h period. RNAs were detected using a ³²P-labeled NF-κB1 specific probe. (B) Total proteins were isolated from CEM/CMV or CEM/AsEgr-1 cells after the addition of either PMA/PHA or TNF-α. Proteins were subjected to PAGE and analyzed using an Egr-1–specific antibody and enhanced chemiluminescent assay. NS, shows the presence of a nonspecific band and demonstrates that equal amounts of protein were loaded in each lane.

Egr-1 is required for endogenous NF-κB1 gene expression. (A) Total RNAs were isolated from CEM/CMV control cells or from CEM/AsEgr-1 cells stimulated with either PMA/PHA or TNF-α over a 4 h period. RNAs were detected using a ³²P-labeled NF-κB1 specific probe. (B) Total proteins were isolated from CEM/CMV or CEM/AsEgr-1 cells after the addition of either PMA/PHA or TNF-α. Proteins were subjected to PAGE and analyzed using an Egr-1–specific antibody and enhanced chemiluminescent assay. NS, shows the presence of a nonspecific band and demonstrates that equal amounts of protein were loaded in each lane.