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. 2003 Nov;71(11):6426–6434. doi: 10.1128/IAI.71.11.6426-6434.2003

FIG. 2.

FIG. 2.

Detection of the pyrG-kdsA-eno operon by RT-PCR analysis using total RNA isolated from strain 7169 (top panel) or 7169kdsA11 (bottom panel). Refer to Fig. 1 and its legend for the annealing position and polarity of each gene-specific primer. The RT-PCRs were performed using the following nucleic acid templates: lanes 1, strain 7169 chromosomal DNA; lanes 2, total RNA isolated from 7169; lanes 6, strain 7169kdsA11 chromosomal DNA; lanes 7, total RNA purified from 7169kdsA11. Reaction sets contained the following primers: a, 381 and 382; b, 383 and 384; c, 385 and 386; d, 387 and 388; e, 389 and 390. The results for one representative set of control reactions (performed using primers 385 and 386) are depicted on the agarose gels as follows: lanes 3 and 8, RT-PCRs using purified total RNA as the nucleic acid template but without activation of the RT; lanes 4 and 9, RT-PCR with no added nucleic acid template; lanes 5 and 10, RT-PCR using chromosomal DNA as the template. Molecular size standards (lanes M) are depicted in 100-bp increments.