TABLE 1.
Nucleotide sequence of oligonucleotide primers used for PCR-based cloning procedures and RT-PCR analyses
| Primer | Sequence (5′-3′) |
|---|---|
| 316 | ATCCTTGGTTTGTGGCGGTG |
| 317 | AACATCTGCTTGAATGGTTGG |
| 334 | GGATCCGTTACGCACGCACTTCAAGAGCa |
| 335 | TTGGGTTGCCACGAGAATCC |
| 341 | CCGCGGGGGTGACTAACTAGGAGGAATAAATGGCTAb |
| 342 | GGATCCGTCGACTCTAGAGGATCCCCGGGTCATTAa |
| 343 | CCATGGTCACCCAGAGTTTACCAGCc |
| 344 | CCGCGGGCCAAGTCAAGCCATTCTCb |
| 381 | CGATTTTGGTTCCAGGTGGC |
| 382 | CATCAGAGCGGGTTTGAAGTTC |
| 383 | TGATAGTGATTTGGGCGGCAC |
| 384 | AAGGCTTGGACCACGAAAAGAG |
| 385 | GGCGGTATGAATGTGCTTGAAAG |
| 386 | GCTCTTGAAGTGCGTGCGTAAC |
| 387 | TTACGCACGCACTTCAAGAGC |
| 388 | TTGGGTTGCCACGAGAATCC |
| 389 | AACGCCAATAGCCAAATCCG |
| 390 | CCGACAAATCAAAACCCTTACCAC |
a
Engineered BamHI site is underlined.
b
Engineered SacII site is underlined.
c
Engineered NcoI site is underlined.