Table I.
Karyotypic Analysis of Pro-B and T Cell Lymphomas
| Locus involvement |
Amplification
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Tumor no. |
Genotype | Immunophenotype | Composite karyotype | TCR | IgH | Age of onset |
IgH | c-myc |
| L796 | Ku80−/−ATM+/+ | Thymic lymphoma | 39,X,-X, or Y, T(2:13)(B;C1),Rb(7.15),T(13;2)(B;B),+15 | No | n.d. | 7 mo | No | No |
| L2282 | Ku80−/−ATM+/+ | Thymic lymphoma | 40,XX.T(3;14)(H4;E3) | No | n.d. | 6 mo | No | No |
| L2283 | Ku80−/−ATM+/+ | Thymic lymphoma | 40,XX,T(7;14)(B;D),Rb(13.17),T(14;7)(C;C),+15 | No | n.d. | 13 mo | No | No |
| L387 | Ku80−/−ATM+/− | Thymic lymphoma | 42,XY,del(12)(F),+Rb(14.14),-14[2],+15 | No | n.d. | 7 mo | No | No |
| L2240 | Ku80−/−ATM+/− | Thymic lymphoma | 40,XX,Rb(5,18)[1],Rb(13.14)[1],Rb(15.15),+15 | No | n.d. | 8 mo | No | No |
| L3835 | Ku80−/−ATM+/− | Thymic lymphoma | 42,X,T(X;4)(F;C),del(13)(A2→A5),+15,+T(15;1)(C;D) | No | n.d. | 7 mo | No | No |
| PKT1 | Ku80−/−p53−/− | Pro-B cell lymphoma | 39–49,XY,+1,+5,T(12;15;3;[1]) OR T(12;15;3)x2,+14 | n.d. | Yes | 2 mo | Yes | Yes |
| PKT2 | Ku80−/−p53−/− | Pro-B cell lymphoma | 39–41,XX,+3,T(12;15),dup(15) | n.d. | Yes | 2 mo | Yes | Yes |
| PKT3 | Ku80−/−p53−/− | Pro-B cell lymphoma | 38–42,XY,T(12;15),T(15;12) OR T(15;12;16) | n.d. | Yes | 2 mo | Yes | Yes |
| PKT4 | Ku80−/−p53−/− | Pro-B cell lymphoma | 39–40,XY,T(12;15),T(15;12),dup(16) | n.d. | Yes | 2 mo | Yes | Yes |
| PKT5–13 | Ku80−/−p53−/− | Pro-B cell lymphoma | n.d. | n.d. | Yes | 2 mo | Yes | Yes |
| PK7T1 | Ku70−/−p53−/− | Pro-B cell lymphoma | T(12;15),T(15;12;?) | n.d. | Yes | 2 mo | Yes | Yes |
| PK7T2 | Ku70−/−p53−/− | Pro-B cell lymphoma | T(12;15) | n.d. | Yes | 3 mo | Yes | Yes |
| PK7T3 | Ku70−/−p53−/− | Pro-B cell lymphoma | T(12;15),T(15;12;?) | n.d. | Yes | 3 mo | Yes | Yes |
| PK7T4 | Ku70−/−p53−/− | Pro-B cell lymphoma | n.d. | n.d. | Yes | 2 mo | Yes | Yes |
| N10527 | Ku80−/−p53−/−RAD54−/− | Pro-B cell lymphoma | T(12;15),T(15;12;3) | n.d. | Yes | 1.5 mo | Yes | Yes |
| N9984-B | Ku80−/−p53−/−RAD54−/− | Pro-B cell lymphoma | T(12;15) | n.d. | Yes | 3 mo | No | No |
| N9984-T | Ku80−/−p53−/−RAD54−/− | Thymic lymphoma | T(12;15),T(14;16),Rb(7.18) | No | n.d. | 3 mo | No | No |
| N10238 | Ku80−/−p53+/−RAD54−/− | Thymic lymphoma | T(4;14),T(14;4),T(12;13) | No | n.d. | 3 mo | No | No |
| N10449 | Ku80−/−p53+/−RAD54−/− | Thymic lymphoma | n.d. | n.d. | n.d. | 4 mo | No | No |
| N9454 | Ku80−/−p53−/−RAG−/− | Thymic lymphoma | Polyploid, aneuploid | No | n.d. | 3 mo | No | No |
| N8839 | Ku80−/−p53−/−RAG−/− | Thymic lymphoma | n.d. | n.d. | n.d. | 3 mo | No | No |
| N10611 | Ku80−/−p53−/−RAG−/− | Thymic lymphoma | Polyploid, aneuploid | No | n.d. | 3 mo | No | No |
| N10184 | Ku80−/−p53−/−RAG−/− a | Thymic lymphoma | Polyploid, aneuploid | No | n.d. | 3 mo | No | No |
| N9700 | Ku80+/−p53−/−RAG−/− a | Thymic lymphoma | Polyploid, aneuploid | No | n.d. | 4 mo | No | No |
| N10214 | Ku80+/−p53−/−RAG−/− a | Thymic lymphoma | Polyploid, aneuploid | No | n.d. | 5 mo | No | No |
The immunophenotype as determined by FACS® analysis is indicated for each of the tumors. Due to the instability of some of the tumors, composite karyotypes represent the most commonly observed clonal aberrations by SKY. Due to the high chromosome instability and polyploid nature of the Ku/p53/RAG tumors, it was not possible to derive a representative composite karyotype. Ku70−/−p53−/− tumors were analyzed with painting probes specific for chromosomes 12 and 15. Columns labeled TCR and IgH refer to the involvement of these loci in chromosome translocations as determined by SKY and/or gene specific (Tcrα, Tcrβ, Ig VH, and Ig Cα) BAC probes. n.d., not determined.
Mice additionally expressing a rearranged T cell receptor transgene (AND). The AND transgene did not change the age of onset, the type of tumor or karyotype.