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. 2002 Aug 19;196(4):447–457. doi: 10.1084/jem.20020052

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — Transcription of IDO-gene in eukaryotic cells infected with recombinant IDO-adenoviruses. mRNA was extracted from human embryonic retinoblast cells infected with IDO-adeno-GFP, IDO-adeno, or with replication-defective adenoviruses (neg. ctr.-a) and reverse transcribed into cDNA. PCR with IDO-specific primers was performed and the products analyzed by agarose gel electrophoresis. Positive control consisted of material extracted from recombinant IDO-adenoviruses and neg. ctr.-b of water instead of template. Lane 1: DNA molecular weight marker; lane 2: pos. ctr., 3, neg. ctr.-b; lane: 4, IDO-adeno-GFP; lane 5: IDO-adeno; lane 6: neg. ctr.-a; lane 7: DNA molecular weight marker. Lanes 4 and 5 show the relevant IDO-specific bands.