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Dose-response titration for the SLO-induced production of TNF-α. Mast cells were stimulated for 1 h in the presence of various concentrations of SLO as indicated. Thereafter, biologically active TNF-α was determined in the supernatants (secreted) and in the cell lysates (intracellular) obtained by freezing and thawing. Shown are the means of three experiments. The error bars represent standard deviations.
