FIG. 5.

Treatment of mast cells with SLO induces the transcription of various cytokines. Cells were left untreated (Ø) or incubated with SLO (0.5 μg/ml) or the SLO mutant N402E (0.5 μg/ml) for 1 h, and reverse transcription-PCR was performed as described in Materials and Methods. The experiment shown is representative of three independent experiments with equivalent results. HGPRT, hypoxanthine-quanine phosphoribosyltransferase.