FIG. 8.

Depletion of PKC reduces the SLO-induced production of TNF-αbut does not affect the degranulation of mast cells.(A)After overnight preincubation with PMA, mast cells were activated with SLO (1 μg/ml) for 1 h,and biologically active TNF-αwas determined in the supernatants(Secreted TNF-α)and in the cell lysates(Intracellular TNF-α).(B)After overnight preincubation with PMA, mast cells were activated with SLO (1 μg/ml) for 30 min,and degranulation was measured as the release of β-hexosaminidase. Shown are the meansof threeexperiments. The error bars represent standard deviations.