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. 2003 Jul 7;198(1):173–181. doi: 10.1084/jem.20030446

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Lymphocyte responses induced against GD3 in immunized mice produce IL-4. Mice were immunized with 2 × 10⁶ SK-MEL-28 cells mixed with CFA on day 0, and boosted with 2 × 10⁶ SK-MEL-28 cells mixed with IFA on day 7. Control mice (Freund's adjuvant only) were injected with CFA on day 0 followed by IFA alone on day 7. (a) Splenocytes were collected on day 14 and stimulated with irradiated syngeneic APCs loaded with GD3, GM2, or without ganglioside (None) as indicated. IL-4 and IFN-γ production were detected by ELISPOT assay. (b) In other sets of mice, hepatic mononuclear cells and thymocytes were tested for IL-4 secretion. Data represent mean ± SEM of triplicate wells from four mice and similar results were observed in repeated experiments.