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. 2003 Jul 7;198(1):91–98. doi: 10.1084/jem.20030615

Figure 5.

Figure 5.

Characterization of pre-B cell tumors by flow cytometry. (A) Dot plots for cytoplasmic SLC and μ H chain in gated B220+ cells from tumor samples from the indicated mice, grown for 1 to 3 wk in the presence of IL-7. (B) Flow cytometric analysis of lacZ expression in gated CD19+B220+ pre-B lymphoma cells in a lymph node from a Btk+/−SLP-65−/− mouse. (C) Phenotype of two pre-B cell lymphoma cultures, showing variable expression of cytoplasmic κ L chain, CD43, and CD2. Cell suspensions were stained for the indicated markers in combination with B220, and the results are displayed as dot plots of gated B220+ cells. (D) Phenotype of two separate tumor cell suspensions derived from BM and mesenteric lymph node from a single mouse, which were cultured in the presence of Il-7 for 7 d.