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. 2003 Jul 7;198(1):39–50. doi: 10.1084/jem.20022235

Figure 5.

Figure 5.

The effect of Kupffer cells and LSECs on proliferation of activated T cells. In vitro–activated PD-1−/− or WT T cells (3 × 104 cells/well) were cocultured for 48 h with or without mitomycin C–treated LNPCs (before separation, 2 × 105 cells/well, consisting of 20% Kupffer cells and 80% LSECs), purified KCs alone (4 × 104 cells/well), purified LSECs alone (1.6 × 105 cells/well), or mixture of purified Kupffer cells + LSECs (4 × 104 cells: 1.6 × 105 cells/well). T cell proliferation was measured by BrdU incorporation.