Figure 3.

Capacity of neutralizing antibodies to CD80 and CD86 to prevent suppression of T cell activation by cultured PE cells. Anti-CD3 stimulation of purified C57BL/6 T cells was performed as described in the legend to Fig. 1. Cultured IPE (A), CBPE (B), or RPE cells (C) were added in the presence or absence of 1 μg/ml anti-CD80 and/or anti-CD86 antibodies. As isotype controls, purified hamster IgG for CD80 and purified rat IgG for CD86 were used. After 72 h of incubation, the cultures were assayed for uptake of [³H]thymidine. Mean counts per minute for triplicate cultures are presented ± SEM. Differences of means of T cell proliferation in the presence of PE cells with and without anti-CD80 or anti-CD86 antibodies are compared. *, P < 0.05; **, P < 0.005.