Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Jul 7;198(1):161–171. doi: 10.1084/jem.20030097

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Detection of mRNA expression of costimulatory molecules in ocular PE. mRNA, extracted from cultured IPE, CBPE, and RPE cells with or without 100 U/ml recombinant IFN-γ (samples 1–6), and from freshly obtained mouse iris, ciliary body, and retina (samples 7–9), was reverse transcribed and amplified by PCR using primers for (A) CD80 (B7-1), (B) CD86 (B7-2), (C) PD-L1 (B7-H1), (D) CD40, and (E) GAPDH. PCR products were electrophoresed in 1.5% agarose gel and visualized by staining with ethidium bromide.