Table I.
Tissues | CD80 (B7-1) | CD86 (B7-2) |
---|---|---|
Corneal epithelium | + | + |
Corneal stroma (keratocytes) | − | − |
Corneal endothelium | − | − |
Iris | + | + |
Ciliary body | + | + |
Lens epithelium | + | + |
Lens fiber cells | − | − |
Neural retina | − | − |
RPE | + | + |
Eyes of BALB/c mice were enucleated, snap frozen, sectioned, placed on glass slides, fixed with acetone, and stained with antibodies to CD80, CD86, and isotype IgG controls, followed by biotin-conjugated sec-ondary anti-antibodies, and then developed with streptavidin/horse-radish peroxidase and the DAB substrate. Specimens were examined by light microscopy. Staining of tissues and cells with DAB is indicated with +.