Table I.
Detection of CD80 and CD86 on Freshly Obtained Ocular Tissues
| Tissues | CD80 (B7-1) | CD86 (B7-2) |
|---|---|---|
| Corneal epithelium | + | + |
| Corneal stroma (keratocytes) | − | − |
| Corneal endothelium | − | − |
| Iris | + | + |
| Ciliary body | + | + |
| Lens epithelium | + | + |
| Lens fiber cells | − | − |
| Neural retina | − | − |
| RPE | + | + |
Eyes of BALB/c mice were enucleated, snap frozen, sectioned, placed on glass slides, fixed with acetone, and stained with antibodies to CD80, CD86, and isotype IgG controls, followed by biotin-conjugated sec-ondary anti-antibodies, and then developed with streptavidin/horse-radish peroxidase and the DAB substrate. Specimens were examined by light microscopy. Staining of tissues and cells with DAB is indicated with +.