Table II.
Detection of CD80 and CD86 on Cultured Ocular PE Cells
| PE cell types | CD80 (B7-1) | CD86 (B7-2) |
|---|---|---|
| Iris | + | + |
| Iris, treated with IFNγ | ++ | ++ |
| Ciliary body | + | − |
| Ciliary body, treated with IFNγ | ++ | + |
| Retina | + | (+)a |
| Retina, treated with IFNγ | ++ | (+)a |
a
Staining within cytoplasmic granules without evidence of cell surface staining.
Extent of staining is presented semiquantitatively as −, +, or ++.
PE cells from iris, ciliary body, and retina were cultured for 14 d, washed, and then incubated overnight in serum-free medium, with or without 100 U/ml IFNγ. The cells were then removed, fixed with acetone, and stained with FITC-conjugated anti-CD80 or anti-CD86 antibodies, and examined by fluorescent microscopy.