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. 1997 Jan 6;185(1):99–110. doi: 10.1084/jem.185.1.99

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MCP-5 mRNA expression in murine leukocytes and endothelial cells. (A) Northern analysis of total RNA isolated from eosinophils purified from the spleens of CD5-IL5 transgenic mice (Eos), bone marrow–derived mast cells either untreated (Ctl) or stimulated with IgE antiTNP and TNP-BSA (IgE), or Con A, resident peritoneal macrophages (RMφ) (93% mononuclear, 7% granulocytes), elicited peritoneal macrophages (EMφ) (76% mononuclear, 24% neutrophils), and elicited peritoneal neutrophils (95% neutrophils, 5% mononuclear). Blots were hybridized with MCP-5, JE, and rpL32 cDNA probes and exposed for 14 d, 3 d, and 18 h, respectively. (B) Northern analysis of total RNA isolated from SVEC cells treated for 6 and 18 h with IFNγ (200 U/ml), IL-4 (10 ng/ml), and IL-1β (5 ng/ml) and 10 μg of total RNA isolated from RAW 264.7 macrophage cell line, either untreated or treated for 18 h with IFNγ (200 U/ml) or the indicated concentrations of LPS. Blots were hybridized sequentially with MCP-5, JE, and rpL32 cDNA probes and exposed for 7 d, 3 d, and 18 h, respectively.

MCP-5 mRNA expression in murine leukocytes and endothelial cells. (A) Northern analysis of total RNA isolated from eosinophils purified from the spleens of CD5-IL5 transgenic mice (Eos), bone marrow–derived mast cells either untreated (Ctl) or stimulated with IgE antiTNP and TNP-BSA (IgE), or Con A, resident peritoneal macrophages (RMφ) (93% mononuclear, 7% granulocytes), elicited peritoneal macrophages (EMφ) (76% mononuclear, 24% neutrophils), and elicited peritoneal neutrophils (95% neutrophils, 5% mononuclear). Blots were hybridized with MCP-5, JE, and rpL32 cDNA probes and exposed for 14 d, 3 d, and 18 h, respectively. (B) Northern analysis of total RNA isolated from SVEC cells treated for 6 and 18 h with IFNγ (200 U/ml), IL-4 (10 ng/ml), and IL-1β (5 ng/ml) and 10 μg of total RNA isolated from RAW 264.7 macrophage cell line, either untreated or treated for 18 h with IFNγ (200 U/ml) or the indicated concentrations of LPS. Blots were hybridized sequentially with MCP-5, JE, and rpL32 cDNA probes and exposed for 7 d, 3 d, and 18 h, respectively.