Table 1.

Membrane TNF Expressing B2.20 Cells Induce Apoptosis but Not HIV Replication in ACH-2 Cells

Treatment		Cell No.*		Apoptotic Cells‡		RT activity§
		×103		%		cpm/μl
None		111		4		38 ± 8
sTNF		93		18		212 ± 20
CHO		125		6		27 ± 5
B2.20		68		28		54 ± 15

ACH-2 cells were seeded at 125 × 10³ cells/well, in the absence or presence of sTNF (30 ng/ml) or in wells containing monolayers of CHO or B2.20 cells as described in Materials and Methods. 22 h after onset of cultures, 10 μl of supernatant was removed and RT activity determined. Thereafter, the nonadherent ACH-2 cells were removed from the wells (60 wells for each experimental condition), pooled, and counted.  

^* Average calculated number of cells recovered per well.  

^‡ The cells were further stained with YOPRO-1™, 200 cells for each group were counted and cells displaying fragmented or condensed nuclei were scored as apoptotic.  

^§ Mean values ± SD of six replicates.